Journal: iScience

Article Title: Intercellular communication between hepatic stellate cells and myofibroblasts mediated by osteopontin and FGF18 promotes liver fibrosis

doi: 10.1016/j.isci.2025.112932

Figure Lengend Snippet: FGF18 primarily targets HSCs, whereas FGF21 mainly induces signals in hepatocytes (A and B) FGF21 increases the expression of Fgf21 and Klb but downregulates the expression of senescence markers, including Cdkn1a and Cdkn2a in hepatocytes. Primary hepatocytes were isolated as in the and stimulated with FGF18 (100 ng/mL) or FGF21 (100 ng/mL) for 4 h (A) or 24 h (B). The expression of the indicated genes was analyzed via qPCR. Results are mean ± SD of triplicate samples. (C and D) FGF21 does not upregulate or downregulate the expression of profibrotic genes. HSCs were isolated as described in A, serum-starved for 24 h, and then left untreated or stimulated with FGF18 (100 ng/mL) or FGF21 (100 ng/mL) for 4 h (C and D) or with FGF21 (100 ng/mL) for 24 h (D). The expression of the indicated genes was analyzed via qPCR. Results are expressed as the means ± SDs of triplicate samples. All results are representative of two independent experiments. Statistical significance was determined by two-tailed unpaired Student’s t tests. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001; ns, not significant.

Article Snippet: Human FGF21 , PeproTech , Cat#100-42.

Techniques: Expressing, Isolation, Two Tailed Test